The trio collaborated on Adam's song 'Shady' for his album Trespassing, but they've never all performed it live together - until now.
Adam was presented with the Unity award at the We Are Family Foundation's Celebration gala at New York's Hammerstein Ballroom, and soon after performed 'Shady' with Nile, Sam and Nile's band CHIC.
Nile had previously described recording the track as "one of the most organically perfect jams I've had since Bowie," and seemed to enjoy performing it live.
Adam received the Unity award for 'spreading peaceful messages through music'.
Adam recently chose to 'donate' his 30th birthday to the We Are Family Foundation.
Fans can pledge money to the organisation, and receive treats if they do so, including recording a birthday message that the star will see.
Below: Adam Lambert performs Matinee Circus show for Hollywood fans